Monday, December 1, 2014

Four Things I Wish I Had Known Before I Started Writing A Manuscript

We all await for those sweet words to come our way, “I think we’re ready to start writing up your data in to a manuscript.”  Finally, you get a chance to start writing up your projects as a first author and can tell the world (or rather the small group of people that study the same thing you do) about all the cool science you’ve been doing.  I was excited when I started to write up my data.  Finally I felt like I was making some progress.  However as I started the manuscript writing process, I realized I was in for an experience.  One that I wish somebody had told me about.  So for right now, I will share some of the major things I’ve learn as I am currently writing my first manuscript as a first author.  Once my manuscript has been sent back (most likely), revised and is published (fingers crossed), I’ll share what else I learned along the way. 

Four things I wish I had known before starting to write a manuscript
1) Ultimately, it your PI’s call as to what goes into your paper.
Even though you did the hands on work and have been actively writing and editing the paper, your PI has the final say.  Their grants funded your work and paid for your salary.  Also, it is their name and reputation on the line when they publish your work.  This may vary from PI to PI as to how much input you get to have on the content of your paper.  I know that I started out with a certain idea of how my work should be written and presented however it was not the best way.  And it can be challenging getting used to having your idea for your work being changed into somebody else’s idea for your work.  I’ve been told that this is part of the process that everybody goes through.  I felt like I was doing something wrong every time the draft came back and was substantially from what I had sent two days before.  Apparently, this is normal.  Crappy, but normal. 

2) Communication between you and your co-authors is really important.
The clearer you and your PI are about the direction of your paper the better.  You both waste less time trying to get on the same page about how to write up your work and which experiments to you need to prioritize to fill in the hole in your story.  I recommend having a meeting with your PI before you start writing so you can start on the same page and then have regular meetings to keep you on schedule with your writing and also to keep communication flowing.

3) It is another full time job – so now you have two: research and writing.  And they are supposed to be done at the same time.
Finding a balance between writing and doing bench work is challenging not to mention balancing your interests outside of lab.  Good luck.  I feel like all grad school is a balancing act.

4) It will take way longer than you think to write the paper.
Even the best planned timeline for getting a draft to co-authors and getting a draft submitted will go awry.  Get used to it.  It gets done when it get done.  Learn from all the delays that come your way.

This was written Kaitlin McDaniel, a 5th year Pathobiology student.

Monday, November 3, 2014

Good Fall Eats on a Graduate Student Budget

Hello CMIID students!

Regardless of our status, first year graduate student, fifth year graduate student, or post doc, we typically live paycheck to paycheck (maybe tossing some money into savings). I know for myself that after rent, utilities, and a car payment, my weekly grocery budget is fairly set. To stretch that budget, I try to shop in season. By that I mean purchasing vegetables and fruits that are currently being harvested and are thus fresh and inexpensive.

We are currently smack dab in the middle of the fall season and there are a plethora of veggies and fruits in season. These include:

Fruits                           Vegetables
Apples                         Arugula
Clementines                 Root veggies (carrots, parsnips, sweet potatoes, turnips)
Cranberries                  Pumpkin
Dates                           Kale and spinach
Figs                              Brussel sprouts
Pears                            Squash (spaghetti, acorn, buttercup, delicata)
                                    Onions
                                    Celery

Local farmers markets obviously have some great deals on produce. The grocery stores are also worth a look though. Wegman’s, for example, will stock some locally grown and produced foods. Trader Joe’s is where I buy a lot of my winter squash. They sell it at a flat rate whereas most other stores sell per pound. It all just takes some willingness to shop around and become familiar with seasonal produce.

And once you’ve got the produce, it all comes down to preparing some tasty dishes; perhaps a hearty soup, roasted squash, or stuffed baked sweet potatoes. Below are a few links to some tasty looking recipes:

Happy shopping and cooking!

Lindsay

Lindsay Snyder is a 4th yr Immunology and Infectious Disease graduate student in Dr. Cantorna's lab.

Wednesday, October 15, 2014

Starting Over: 5 things you didn’t realize would slow you down in a new post doc.


You leave your PhD lab feeling confident because you just spent 4-8 years in your field, where you have read, researched, and written parts of your field.  You believe that you have a strong technical background in what you’ve done, and you may decide to change fields slightly for your post-doc. Doing this opens you up to expanding your knowledge much further than if you maintained a position in your current field, and it also opens you up to feeling stupid most of the time. (See The Importance of Stupidity in Scientific Research: http://jcs.biologists.org/content/121/11/1771.full.pdf+html).

Don’t worry – this is normal.

Just like when you first started graduate school and knew nothing about the field, you will need time to adapt. I changed from a microbiology lab with a focus on the immune response against whooping cough (vaccinate your kids!), to an erythropoiesis lab, learning how the body deals with stresses that cause dramatic loss of red blood cells due to either trauma or leukemia. Because these fields are very different, I expected it to take some time to adapt, but I wasn’t sure how long before I started to really get going. 

Here is what I’ve learned:
1. Paperwork doesn’t normally transfer. Even though I have decided to continue my career at the same institution that I carried out my graduate work in, new paperwork is needed as an employee that was either different because of my change in status from graduate student to employee or did not transfer to my new laboratory. You will be expected to complete the same training you have already completed because you are in a different building, lab, or department. You will need to be added to new IACUCs, IRBs, and IBCs, because these are laboratory specific. This paperwork will take a while.

2. Just because you learned a technique previously doesn’t mean you know how to do it.  Every lab has their own way of doing things. Some may extract bone marrow by drilling into the bone and flushing the marrow out while others may just pulverize the whole bone and filter out the unwanted cells. Neither of these versions are incorrect, but labs have a tendency to maintain their standard operating procedures (SOPs) to make sure that data are comparable among members within the lab. It will take time to learn from the lab members to know how certain procedures and techniques are expected to be performed, which leads to our next point.

3. You will need to work on someone else’s schedule until you have learned those SOPs. When you first start a lab and are trying to learn how that lab does things, you will need to observe other labmates, and even work side by side with them until you can master their SOPs.

4. You will need to learn a new set of jargon and the literature of a new field. This one is pretty obvious. Whether you change course slightly or hop into a brand new field that you know little to nothing about, you will need to learn the update views, big names in the field, and

5. You will need to learn how your new boss operates. How often do you meet? Are you expecting to share data on a specific day at a specific time or should you report new data as it comes in? Is there a regular lab meeting, and if so, what is the format? Will you share or present data every time? Is this a general update on progress? Are important journal articles discussed? How closely related are the journal articles to people’s work? This is very much so lab specific, and every lab has a different way of doing things. The best advice suggests that you talk to labmates to discover how things run and how you should contribute.

For these reasons, it really will take you about 2 months to start being a productive member of the lab when you change field. Most PIs expect this, and although you should be working hard to get yourself deeper into the new field, give yourself a break now and then to step back and realize how much you will have learned when starting over.

This post was written by Dr. Laura Goodfield, post-doc in Dr. Paulson's lab. 


Monday, October 13, 2014

It’s a bird! No…it’s a plane! No… its “The Speed of Trust!”

In August, Rick Parmely of Professional and Polished, LCC visited our CMIID group and mentioned “The Speed of Trust” by Stephen M.R. Covey as part of his professional development talk.  As of late, I’ve been very interested in leaving grad school being well-rounded - that is, having more skills under my belt than flow cytometry and mucosal cell isolations, so I decided to read it.

While there were many good points and interesting ideas from the book, I came away with two points that really made me think.
1: Trust is made of two parts: integrity and capability.  I would trust my lab mate to homogenize the spleens from my experiment but not to perform my open heart surgery.  My doctor could perform the surgery but probably not isolate my lamina propria lymphocytes.

2: The degree of trust is directly proportional to how fast tasks get accomplished and inversely proportional to how much it costs to finish the task.  For example, if trust is low, it can take a long time for a collaboration project to produce data, which will cost more money and result in fewer publications or patents.  But if the trust is high, the collaboration can work quickly to produce results, which will cost less money and result in more frequent publications. 

The majority of the book focuses on how to increase trust within your organization which was interesting to me because as I said before, I had never thought about how trust could be important in the work environment.  “The Speed of Trust” has definitely made a strong case for trust in the workplace.  Trust within personal relationships is also discussed in this book but for this, I’ll stick to the workplace examples.       

While we are all science nerds and therefore notorious for our lack of social skills, we still need to have interpersonal skills in order for our work to run smoothly.  Isn’t it easier to have a lab mate that you trust take care of a small part of your big experiment so that you can focus on the main objective?  Isn’t it easier to communicate with your PI when you trust them to guide you through a rough patch of experiments and when they are confident in your ability to perform the actual science correctly?  Doesn’t the work get done faster when you’re not worried about whether the PBS you made last week hasn’t been swapped out with HCl by one of your lab mates? (I haven’t had any experience with this but it was a horror story somebody told me while I was interviewing at grad schools.)

All of this has made me think about how my actions are perceived in the lab. Do my actions help people trust me?  Do my actions help me trust me?  I believe that people want to be connected to other people and by having integrity and expressing compassion.  I personally feel my best when I’m working positively with others, making a connection with them whether it is through a collaborative experiment or an idea exchange.  When we connect with other, it forms a network of support and a platform for productivity and positivity.  Learning how to build a network and platform now will help us transition into any job opportunities, research based or non-traditional, and any professional and personal relationships we have in the future.

And on that note, what do your actions say about you?  Can your lab trust you to get your work and participate positively as member of the lab community?   Or are you that person that nobody wants to work with and has a reputation for being unreliable?  Are you in the process of building a supportive network?  If you are interested in learning about how trust is a vital part to our lives in and out of the lab, I highly suggest reading “The Speed of Trust.”



“Whether you’re on a sports team, in an office or a member of a family, if you can’t trust one another there’s going to be trouble.” – Joe Paterno

“There are no moral shortcuts in the game of business – or life.  There are, basically three kinds of people: the unsuccessful, the temporarily successful and those who become and remain successful.  The difference is character.”  - Jon Hunstman, Chairman – Huntsman Chemical

“What creates trust, in the end, is the leader’s manifest respect for the followers.” – James O’Toole, Author of Leading Change



This post was written by Kaitlin McDaniel, a 5th yr PhD. Candidate in Dr. Cantorna’s lab.